Lactobacilli Modulate Hypoxia-Inducible Factor (HIF)-1
Regulatory Pathway in Triple Negative Breast
Cancer Cell Line
# The first two authors equally contributed to this manuscript.
Hypoxia-Inducible Factor (HIF)-1 plays an essential role in the body’s response to low oxygen concentrations and regulates expression of several genes implicated in homeostasis, vascularization, anaerobic metabolism as well as immunological responses. Increased levels of HIF-1α are associated with increased proliferation and more aggressive breast tumor development. Lactobacilli have been shown to exert anti-cancer effects on several malignancies including breast cancer. However, the exact mechanism of such effect is not clear yet. The aim of this study was to analyze the expression of selected genes from HIF pathway in a triple negative breast cancer cell line (expressing no estrogen and progesterone receptors as well as HER-2/Neu), MDA-MB-231, following treatment with two lactobacilli culture supernatants.
Materials and Methods
In this experimental study, we analyzed the expression of
Both LRS and LCS had cytotoxic effects on MDA-MB-231 cells, while the former
type was more cytotoxic. LRS dramatically down-regulated expression levels of the
Although both LCS and LRS had cytotoxic effects on the MDA-MB-231 cells,
it is proposed that LRS could be more appropriate for pathway directed treatment modalities, as it did not decrease expression of tumor suppressor genes involved in HIF pathway.
Down-regulation of HIF pathway mediated oncogenes by LRS suggests that the cytotoxic
effects of this