Ps-111: Neural Differentiation of Human Bone Marrow Derived Stem Cells Using Real Time RT-PCR Technique (Pages: 142-142)


Mostafavi FS *, Razavi Sh , Kazemi M ,

0

Objective: Human adult stem cells which are capable of self-renewal and differentiation into other cell types can be isolated from various tissues. There are no ethical and rejection problems as in the case of embryonic stem cells, so they are a promising source for cell therapy. The human body contains a great amount of mesenchymal stem cells (MSCs). For the first time MSCs separated from bone marrow (BM) and between MSCs they are the most important one which are used in researches. The aim of this study was to explore the potential of human BM-derived stem cells (hBMSCs) in differentiating toward neural lineage. Materials and Methods: To isolate hBMSCs, BM aspiration was cultured in DMEM: F12/10% FBS. The expression profiles of several MSC markers were examined by flow cytometry. After neurosphere formation, final differentiation was induced by B27, NEAA, L-glu and N2. Differentiated hBMSCs were evaluated for Nestin and MAP2 expression using Real time RTPCR technique. Results: Our results showed that BM-derived MSCs could be successfully differentiate into neuron-like cells as defined by cell morphology and up-regulation of neural marker MAP-2 expression in compare with undifferentiated cells. Conclusion: Regarding to our results it seems that we can use BM tissue in order to investigate agents which consider having positive expected effects on neural cells’ activities.