Past Issue

Volume 19, Supplement 1, Spring 2017 Pages: 106-112

Continuous or Transient High Level of Glucose Exposure Differentially Increases Coronary Artery Endothelial Cell Proliferation and Human Colon Cancer Cell Proliferation


Yoko Shimoda, M.D., Ph.D., Yuko Tagaya, M.D., Ph.D., Tsugumichi Saito, M.D., Ph.D., Eijiro Yamada, M.D., Ph.D., Aya Osaki, M.D., Ph.D., Yasuyo Nakajima, M.D., Ph.D., Atsushi Ozawa, M.D., Ph.D., Tetsurou Satoh, M.D., Ph.D., Junichi Okada, M.D., Shuichi Okada, M.D., Ph.D., *, Masanobu Yamada, M.D., Ph.D.,
Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Showa-machi, Maebashi, Gunma, Japan
*Corresponding Address: Department of Medicine and Molecular Science Gunma University Graduate School of Medicine 3-39-15 Showa-machi Maebashi Gunma 371-8511 Japan Email:okadash@gunma-u.ac.jp

Abstract

We studied effect of high glucose levels on coronary artery endothelial cell proliferation and human colon cancer cell proliferation. To examine the long-term effect of glucose exposure on cell growth, cells were cultured for 14 days in the absence or presence of 183 mg/dL D-glucose addition in the culture medium. Short effect of elevated glucose levels was examined by addition of 183 mg/dL D-glucose addition in the culture medium for just one hour per day followed by changing the culture to standard medium (5.5 mM D-glucose) during the next 23-hours period. Cell proliferation was estimated by 2,3-Bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carbox-anilide (XTT) assay and phosphor-Erk western blot analysis. We found that coronary artery endothelial cell proliferation was significantly increased in the culture medium with the acute one-hour addition of 183 mg/dL D-glucose compared to the absence or chronic presence of 183 mg/dL D-glucose addition in the culture medium. In contrast, colon cancer cell proliferation was significantly increased in the continuous presence of 183 mg/dL D-glucose addition in the culture medium compared to the acute one-hour addition of glucose. The extent of Erk2 phosphorylation paralleled with the relative changes in cellular proliferation in both cell types. Taken together, these results suggested that continuous or transient high level of glucose exposure differentially effects coronary artery endothelial and human colon cancer cell proliferation.