Inhibition of AGS Cancer Cell Proliferation following siRNA-Mediated Downregulation of VEGFR2

(Pages: 381-388)
Ali Zarei Mahmudabadi, Ph.D, 1Masoomeh Masoomi Karimi, M.Sc, 2Majid Bahabadi, Ph.D, 3Zahra Bagheri Hoseinabadi, Ph.D, 4Moslem JafariSani, Ph.D, 5,*Reza Ahmadi, Ph.D, 6
Department of Biochemical, Chemical Injuries Research Center, Baqiyatallah University of Medical Science, Tehran, Iran
Department of Immunology, Faculty of Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
Department of Biochemistry, Qazvin University of Medical Sciences, Qazvin, Iran
Department of Biochemistry, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Department of Basic Sciences, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
Biochemistry Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Department of Biochemical, Chemical Injuries Research Center, Baqiyatallah University of Medical Science, Tehran, Iran
Department of Immunology, Faculty of Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
Department of Biochemistry, Qazvin University of Medical Sciences, Qazvin, Iran
Department of Biochemistry, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Department of Basic Sciences, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
Biochemistry Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
*Corresponding Address: P.O.Box: 9691739643 Department of Basic Sciences School of Medicine Shahroud University of Medical Sciences Shahroud Iran Email:moslem.jafarisani@gmail.com
Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Zarei Mahmudabadi Ali, Masoomi Karimi Masoomeh, Bahabadi Majid, Bagheri Hoseinabadi Zahra, JafariSani Moslem, Ahmadi Reza. Inhibition of AGS Cancer Cell Proliferation following siRNA-Mediated Downregulation of VEGFR2. Cell J. 2016; 18(3): 381-388.

Abstract

Objective

Vascular endothelial growth factor (VEGF) and VEGF receptors (VEGFRs) play important roles in angiogenesis of different developmental mechanisms such as wound healing, embryogenesis and diseases, including different types of cancer. VEGFR2 is associated with cell proliferation, migration, and vascular permeability of endothelial cells. Blocking VEGF and its receptors is suggested as a therapeutic approach to prevent tumor growth. In this study, we aim to block VEGF signaling via small interfering RNA (siRNA) inhibition of VEGFR2.

Materials and Methods

In this experimental study, we used the RNA interference (RNAi) mechanism to suppress expression of the VEGFR2 gene. We conducted the 3-(4,5-di- methylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay, real-time polymerase chain reaction (PCR), Western blot, and flow cytometry analyses of VEGFR2 expression.

Results

Real-time PCR and Western blot results showed that VEGFR2 expression significantly downregulated. This suppression was followed by inhibition of cell prolifera- tion, reduction of viability, and induction of apoptosis in the cancer cells.

Conclusion

These findings suggest that VEGFR2 has a role in cell proliferation and tumor growth. Accordingly, it is suggested that VEGFR2 can be a therapeutic target for controlling tumor growth and proliferation.