Ameliorating Effect of Ginseng on Epididymo-Orchitis
Inducing Alterations in Sperm Quality and
Spermatogenic Cells Apoptosis following
Infection by Uropathogenic
Escherichia coli in Rats
Epididymo-orchitis (EO) potentially results in reduced fertility in up to 60% of affected patients. The anti-inflammatory effects of Korean red ginseng (KRG) and its ability to act as an immunoenhancer in parallel with the beneficial effects of this ancient herbal medicine on the reproductive systems of animals and humans led us to evaluate its protective effects against acute EO.
Materials and Methods
This animal experimental study was conducted in the Department of Anatomical Sciences,
Faculty of Medicine, Zanjan University of Medical Sciences
(ZUMS), Zanjan, Iran during 2013-2015. We divided 50 Wistar rats into five following
groups (n=10 per group): i. Control-intact animals, ii. Vehicle-phosphate buffered saline
(PBS) injection into the vas deferens, iii. KRG-an intraperitoneal (IP) injection of KRG, iv.
EO-an injection of uropathogenic
Acute EO increased the relative weight of prostate and seminal vesicles (P≤0.05). It also reduced sperm quality such as total motility, sperm concentration (P≤0.01), and the percentage of normal sperm (P≤0.001). Moreover, acute EO decreased Miller’s (P≤0.05) and Johnsen’s scores and increased apoptotic indexes of spermatogenic cells (P≤0.001). KRG treatment decreased prostate weight gain (P≤0.05) and improved the percentage of sperm with normal morphology, total motility (P≤0.01), and progressive motility (P≤0.05). The apoptotic indexes of spermatogenic cells reduced (P≤0.001), whereas both Johnsen’s (P≤0.01) and Miller’s criteria increased in the KRG-treated EO testis (P≤0.05).
Consequently, KRG ameliorated the devastating effects of EO on the sperm retrieved from either epididymis or testicle in rats.