Indoleamine 2,3-Dioxygenase Is Dispensable for The Immunomodulatory Function of Stem Cells from Human Exfoliated Deciduous Teeth

(Pages: 597-608)
Razieh Alipour, M.Sc, 1Masoumeh Masoumi Karimi, M.Sc, 2Batool Hashemi-Beni, Ph.D, 1Minoo Adib, Ph.D, 1,*Nasrin Sereshki, M.Sc, 1Farzaneh Sadeghi, M.Sc, 1
Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
School of Medicine, Shahroud University of Medical Science, Shahroud, Iran
Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
School of Medicine, Shahroud University of Medical Science, Shahroud, Iran
*Corresponding Address: P.O.Box: 81744-176 Department of Immunology Medical School Isfahan University of Medical Sciences Hezar Jerib Street Isfahan Iran Email:adib@med.mui.ac.ir
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Alipour Razieh, Masoumi Karimi Masoumeh, Hashemi-Beni Batool, Adib Minoo, Sereshki Nasrin, Sadeghi Farzaneh. Indoleamine 2,3-Dioxygenase Is Dispensable for The Immunomodulatory Function of Stem Cells from Human Exfoliated Deciduous Teeth. Cell J. 2017; 18(4): 597-608.

Abstract

Objective

In this study, we sought to better understand the immunoregulatory function of stem cells derived from human exfoliated deciduous teeth (SHED). We studied the role of the interferon gamma (IFN-γ)-indoleamine 2,3-dioxygenase (IDO)-axis in immunoregulation of SHED compared to bone marrow derived mesenchymal stem cells (BMMSCs) under the same conditions.

Materials and Methods

In this cross-sectional study, recently isolated human T cells were stimulated either by mitogen or inactivated allogeneic peripheral blood mononuclear cells (PBMCs). These T cells were subsequently co-cultured with, either SHED or BMMSCs in the presence or absence of 1-methyl-tryptophan (1-MT) or neutralizing anti- human-IFN-γ antibodies. In all co-cultures we evaluated lymphocyte activation as well as IDO activity.

Results

SHED, similar to conventional BMMSCs, had anti-proliferative effects on stimulated T cells and reduced their cytokine production. This property of SHED and BMMSCs was changed by IFN-γ neutralization. We detected IDO in the immunosuppressive supernatant of all co-cultures. Removal of IDO decreased the immunosuppression of BMMSCs.

Conclusion

SHED, like BMMSCs, produced the IDO enzyme. Although IFN-γ is one of inducer of IDO production in SHED, these cells were not affected by IFN-γ in the same manner as BMMSCs. Unlike BMMSCs, the IDO enzyme did not contribute to their immunosuppression and might have other cell-type specific roles.