Differentiation of Human Scalp Adipose-Derived Mesenchymal Stem
Cells into Mature Neural Cells on Electrospun Nanofibrous
Scaffolds for Nerve Tissue Engineering Applications
This study aimed to isolate and culture SADS cells, investigate their neurogenic capacity and evaluate their application for nerve tissue engineering.
Materials and Methods
In this experimental study, SADS cells were isolated from human adipose tissue. After 7-day treatment of SADS cells with insulin, indomethacin and isobutylmethylxanthine, neurogenic differentiation of SADS cells was investigated. During this study, Poly (ε-caprolactone) (PCL) and PCL/gelatin nanofibrous scaffolds were fabricated using electrospinning and subsequently nanofibrous scaffolds were coated with platelet-rich plasma (PRP). SADS cells were also seeded on nanofibrous scaffolds and neurogentic differentiation of these cells on nanofibers was also evaluated. Effect of PRP on proliferation and differentiation of SADS cells on scaffolds was also studied.
Our results showed that after 7-day treatment of SADS cells with insulin, indomethacin and
isobutylmethylxanthine, SADS cells expressed markers characteristic of neural cells such as nestin and neuron specific
nuclear protein (
Our results demonstrated that SADS cells have potential to differentiate into early and mature progenitor