Direct Coculture of Human Chondrocytes and Synovium-Derived
Stem Cells Enhances In Vitro Chondrogenesis
Coculture of chondrocytes and mesenchymal stem cells (MSCs) has been developed as a strategy to
overcome the dedifferentiation of chondrocytes during
Materials and Methods
In this experimental study, passage 2 chondrocytes and SDSCs were directly cocultured
using different ratios of chondrocytes to SDSCs (3:1, 1:1, or 1:3). glycosaminoglycan (GAG) synthetic activity was
assessed using GAG assays and Safranin-O staining. Expression of chondrogenesis-related genes (
GAG/DNA ratios in 1:1 and 1:3 coculture groups were significantly increased compared to those in the
chondrocyte and SDSC monoculture groups.
Direct coculture of human chondrocytes and SDSCs significantly enhanced chondrogenic potential, especially at a ratio of 1:1, compared to chondrocyte or SDSC monocultures.