Brief Azacytidine Step Allows The Conversion of Suspension Human Fibroblasts into Neural Progenitor-Like Cells

(Pages: 153-158)
Fahimeh Mirakhori, M.SC, 1,2Bahman Zeynali, Ph.D, 1Sahar Kiani, Ph.D, 2Hossein Baharvand, Ph.D, 2,3,*
School of Biology, College of Science, University of Tehran, Tehran, Iran
Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran
School of Biology, College of Science, University of Tehran, Tehran, Iran
Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran
*Corresponding Address: P.O.Box: 16635-148 Department of Stem Cells and Developmental Biology at Cell Science Research Center Royan Institute for Stem Cell Biology and Technology ACECR Tehran Iran Email:Baharvand@RoyanInstitute.org
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Mirakhori Fahimeh, Zeynali Bahman, Kiani Sahar, Baharvand Hossein. Brief Azacytidine Step Allows The Conversion of Suspension Human Fibroblasts into Neural Progenitor-Like Cells . Cell J. 2015; 17(1): 153-158.

Abstract

In recent years transdifferentiation technology has enabled direct conversion of human fibroblasts to become a valuable, abundant and accessible cell source for patient-specific induced cell generation in biomedical research. The majority of transdifferentiation approaches rely upon viral gene delivery which due to random integration with the host genome can cause genome instability and tumorigenesis upon transplantation. Here, we provide a simple way to induce neural progenitor-like cells from human fibroblasts without genetic manipulation by changing physicochemical culture properties from monolayer culture into a suspension in the presence of a chemical DNA methyltransferase inhibitor agent, Azacytidine. We have demonstrated the expression of neural progenitor-like markers, morphology and the ability to spontaneously differentiate into neural-like cells. This approach is simple, inexpensive, lacks genetic manipulation and could be a foundation for future chemical neural transdifferentiation and a safe induction of neural progenitor cells from human fibroblasts for clinical applications.