Comparison of The Melatonin Preconditioning Efficacy between
Bone Marrow and Adipose-Derived Mesenchymal Stem Cells
Mesenchymal stem cells (MSC) from various sources have the potentials to positively affect regenerative medicine. Furthermore, pre-conditioning strategies with different agents could improve the efficacy of cell therapy. This study compares the effects of an anti-inflammatory and antioxidant agent, melatonin, on protection of bone marrow-derived MSCs (BMSCs) and adipose tissue-derived MSCs (ADSCs).
Materials and Methods
In this experimental study, rat BMSCs and ADSCs were isolated and expanded. Pre-conditioning
was performed with 5 µM melatonin for 24 hours. Cell proliferation and viability were detected by MTT assay. Expression of
No significant increase was found in cell viability between BMSCs and ADSCs after melatonin preconditioning.
Following melatonin preconditioning,
Here we have shown that the effects of preconditioning on melatonin expression in ADSCs are higher than those in BMSCs. These findings could be used in adoption of a proper preconditioning protocol based on the sources of MSCs in specific clinical applications, especially in bone regeneration.