Anacardic Acid Reduces Acetylation of H4K12 in Mouse
Oocytes during Vitrification
Over the last years, vitrification has been widely used for oocyte cryopreservation, in animals and humans; however,
it frequently causes minor and major epigenetic modifications. The effect of oocyte vitrification on levels of acetylation of
histone H4 at lysine 12 (AcH4K12), and histone acetyltransferase (
Materials and Methods
In this experimental study, 248 mouse oocytes at metaphase II (MII) stage were divided in three
experimental groups namely, fresh control oocytes (which were not affected by vitrification), frozen/thawed oocytes (vitrified)
and frozen/thawed oocytes pre-treated with AA (treatment). Out of 248 oocytes, 173 oocytes were selected and from them,
84 oocytes were vitrified without AA (vitrified group) and 89 oocytes were pretreated with AA, and then vitrified (treatment
group). Fresh MII mouse oocytes were used as control group.
The present study suggests that AA reduces vitrification risks caused by epigenetic modifications, but does not affect the quality of vitrification. In fact, AA as a Hat inhibitor was effective in reducing the acetylation levels of H4K12.