Detection of Mycoplasma Contamination of Cell Culture by A
Loop-Mediated Isothermal Amplification Method
Mycoplasmas are major contaminants of cell culture and affect
Materials and Methods
In this descriptive laboratory study, for LAMP detection of Mycoplasma contaminations in cell
culture, we used primers specifically designed for targeting the 16S rRNA conserved gene of
In this study, the method developed for detection of Mycoplasma contamination of cell cultures was a rapid, sensitive and cost-effective LAMP approach. The results demonstrated that this method benefits from high specificity (100%) for amplification of Mycoplasma strains and high speed (multiplication within 60 minutes), while it does not require expensive laboratory equipment compared to those needed for polymerase chain reaction (PCR)-based detection.
Our study is the first report about application of LAMP assay based on 16S rRNA gene for detection of Mycoplasma strains; this technique could be considered a useful tool for rapid detection of contamination of cell culture.