Dynamics of The Expression of Pluripotency and Lineage Specific
Genes in The Pre and Peri-Implantation Goat Embryo
HosseinNia P, Hajian M, Jafarpour F, Hosseini SM, Tahmoorespur M, Nasr-Esfahani MH. Dynamics of the expression of pluripotency and lineage specific genes in the pre and peri-implantation goat embryo. Cell J. 2019; 21(2): 194-203. doi: 10.22074/cellj.2019.5732.
Two critical points of early development are the first and second lineage segregations, which are regulated by a wide spectrum of molecular and cellular factors. Gene regulatory networks, are one of the important components which handle inner cell mass (ICM) and trophectoderm (TE) fates and the pluripotency status across different mammalian species. Considering the importance of goats in agriculture and biotechnology, this study set out to investigate the dynamics of expression of the core pluripotency markers at the mRNA and protein levels.
Materials and Methods
In this experimental study, the expression pattern of three pluripotency markers (
Relative levels of mRNA transcripts for Nanog and Sox2 in D3 (8-16 cell) embryos were significantly higher
than D7 blastocysts and mature oocytes, while