Panax ginseng Extract Improves Follicular Development after Mouse Preantral Follicle 3D Culture


Abbas Majdi Seghinsara, Ph.D, 1Hamed Shoorei, Ph.D, 1,2,*Mohammad Mehdi Hassanzadeh Taheri, Ph.D, 2Arash Khaki, D.V.M, Ph.D., 3Majid Shokoohi, M.Sc., 1Moloud Tahmasebi, M.Sc., 4Amir Afshin Khaki, Ph.D., 1Hossein Eyni, M.Sc., 4Sadegh Ghorbani, M.Sc., 4Khadijeh Riahi Rad, Ph.D., 5Hossein Kalarestaghi, Ph.D., 6Leila Roshangar, Ph.D., 7
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Anatomical Sciences, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
Department of Pathology, Islamic Azad University, Tabriz Branch, Tabriz, Iran
Department of Anatomical Sciences, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
Department of Horticulture Science, Tarbiat Modares University, Tehran, Iran
Research laboratory for Embryology and Stem Cells, Department of Anatomical Sciences and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Anatomical Sciences, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
Department of Pathology, Islamic Azad University, Tabriz Branch, Tabriz, Iran
Department of Anatomical Sciences, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
Department of Horticulture Science, Tarbiat Modares University, Tehran, Iran
Research laboratory for Embryology and Stem Cells, Department of Anatomical Sciences and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Address: P.O.Box: 5166614766 Department of Anatomical Sciences Faculty of Medicine Tabriz University of Medical Sciences Tabriz Iran Email:h.shoorei@gmail.com
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Majdi Seghinsara Abbas, Shoorei Hamed, Hassanzadeh Taheri Mohammad Mehdi, Khaki Arash, Shokoohi Majid, Tahmasebi Moloud, Khaki Amir Afshin, Eyni Hossein, Ghorbani Sadegh, Riahi Rad Khadijeh, Kalarestaghi Hossein, Roshangar Leila. Panax ginseng Extract Improves Follicular Development after Mouse Preantral Follicle 3D Culture. Cell J. 2019; 21(2): 210-219.

Abstract

Objective

Panax ginseng is a popular traditional herb that has been used in complementary and alternative medicine in eastern Asia, and it possesses pharmacologically active compounds like ginsenosides (GSs). This study aimed to investigate the impact of Panax ginseng extract (PGE) at different concentrations on in vitro follicular function and development in a three-dimensional (3D) culture system fabricated using sodium alginate after 12 days of culture.

Materials and Methods

In this experimental study, preantral follicles (n=661) were mechanically isolated from the ovaries of 14-day-old female NMRI mice using 29-gauge insulin syringes. Follicles were individually capsulated within sodium alginate, and divided into four groups including control and experimental groups 1, 2, and 3. Then, they were cultured for 12 days in the medium supplemented with different concentrations of PGE (0, 50, 100, and 500 µg/ mL, for control groups and groups 1, 2 and 3, respectively). At the end of the culture period, the mean diameter and maturation of follicles, follicular steroid production, mRNA expression level of proliferating cell nuclear antigen (PCNA) and follicle stimulating hormone receptor (FSH-R), and reactive oxygen species (ROS) levels in collected metaphase-II (MII) oocytes were determined.

Results

The mean diameter of follicles in group 2 was significantly increased as compared to other groups (P<0.001). The percentages of the survival and maturation rate and levels of secreted hormones were higher in group 2 than the other groups (P<0.05). Follicles cultured in the presence of PGE 100 µg/mL had higher levels of proliferation cell nuclear antigen (PCNA) and follicle stimulating hormone receptor (FSH-R) mRNA expression in comparison to other groups (P<0.05). Moreover, oocytes collected from groups 2 and 3 had lower levels of ROS as compared to other groups (P<0.05).

Conclusion

Our results suggest that PGE at the concentration of 100 µg/mL induces higher follicular function and development in the 3D culture system.