Evaluation of Tumor Regulatory Genes and Apoptotic Pathways in
The Cytotoxic Effect of Cytochalasin H on Malignant Human
Glioma Cell Line (U87MG)
Heidarzadeh S, Motalleb Gh, Zorriehzahra MJ. Evaluation of tumor regulatory genes and apoptotic pathways in the cytotoxic effect of cytochalasin H on malignant human glioma cell line (U87MG). Cell J. 2019; 21(1): 62-69. doi: 10.22074/cellj.2019.5948.
The aim of current study was to provide a proof-of-concept on the mechanism of
Materials and Methods
In the present experimental study, we have examined cytochalasin H cytotoxic activities as a
new therapeutic agent on U87MG cells
MTT assay showed that cytochalasin H (10-5 M) inhibited the U87MG cancer cells proliferation after 48 hours.
Analysis of qRT-PCR showed that the
This study shows the effect of caspase-independent pathways of the programmed cell death on the U87MG cancer cell line under cytochalasin H treatment. Further studies are needed to explore the exact mechanism.