Preparation and Evaluation of A Novel Liposomal Nano-Formulation in Metastatic Cancer Treatment Studies


Fatemeh Barzegari Firouzabadi, Ph.D, 1,2,*Shahrbanoo Oryan, Ph.D, 1Mohammad Hasan Sheikhha, Ph.D, 3Seyed Mehdi Kalantar, Ph.D., 3Ameneh Javed, Ph.D, 4
Department of Animal Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran
Departeman of Biology, College of Science, Payame Noor University, Yazd, Iran
Reproductive and Genetic Unit, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Department of Biology, Faculty of Science, Science and Art University, Yazd, Iran
Department of Animal Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran
Departeman of Biology, College of Science, Payame Noor University, Yazd, Iran
Reproductive and Genetic Unit, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Department of Biology, Faculty of Science, Science and Art University, Yazd, Iran
*Corresponding Address: P.O.Box: 15719-14911 Department of Animal Biology Faculty of Biological Science Kharazmi University Tehran Iran Email:f.barzegary@gmail.com
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Barzegari Firouzabadi Fatemeh, Oryan Shahrbanoo, Sheikhha Mohammad Hasan, Kalantar Seyed Mehdi, Javed Ameneh. Preparation and Evaluation of A Novel Liposomal Nano-Formulation in Metastatic Cancer Treatment Studies. Cell J. 2019; 21(2): 135-142.

Abstract

Objective

Today, in clinical trials, we suffer from the lack of effective methods with minimal side effects to deliver medication. Thus, efforts to identify better conditions for delivery of biomedical drugs seem necessary. The purpose of this study was to design a new liposomal formula for transportation of microRNA in osteosarcoma.

Materials and Methods

In this experimental study, several liposomal formulations were synthesized. Physical and chemical parameters, including size, zeta potential, polydispersity index, long-term stability of the liposomal-microRNA complex and the amount of miR-143 loading in liposome based nano-vesicles were optimized using different techniques. Similarly, the effect of free and encapsulated microRNA toxicity were investigated and compared in a human bone osteosarcoma cell line, named SaOs-2.

Results

In this study, we could produce a novel and optimized formulation of cationic PEGylated liposomal microRNA for gene delivery. The present synthesized microRNA lipoplex system was non-agglomerated. The system remained stable after four months and miR-143 leakage was not observed by performing gel electrophoresis. The microRNA lipoplex could enhance conduction of the loaded miR-143, and it also showed good biocompatibility to the healthy cells.

Conclusion

The PEGylated microRNA lipoplex system had a high potential for the systematic migration of miR-143 and it could improve intracellular stability of the released microRNA.