Spermatogenesis Recovery Potentials after Transplantation of Adipose
Tissue-Derived Mesenchymal Stem Cells Cultured with Growth
Factors in Experimental Azoospermic Mouse Models
Eliyasi Dashtaki M, Hemadi M, Saki G, Mohammadiasl J, Khodadadi A. Spermatogenesis recovery potentials after transplantation of adipose tissue-derived mesenchymal stem cells cultured with growth factors in experimental azoospermic mouse models. Cell J. 2020; 21(4): 401-409. doi: 10.22074/ cellj.2020.6055.
Approximately 1% of the male population suffers from obstructive or non-obstructive azoospermia. Previous
Materials and Methods
This was an experimental study in which AT-MSCs were first isolated from male Naval Medical Research Institute (NMRI) mice. Then, the mice underwent testicular torsion-detorsion surgery and received bromodeoxyuridine (BrdU)-labeled AT-MSCs into the lumen of seminiferous tubules. The transplanted cells had been cultured in different conditioned media, containing the three growth factors and without them. The expression of germ cell-specific markers was evaluated with real-time polymerase chain reaction (PCR) and western-blot. Moreover, immunohistochemical staining was used to trace the labeled cells.
The number of transplanted AT-MSCs resided in the basement membrane of seminiferous tubules significantly increased after 8 weeks. The expression levels of Gcnf and Mvh genes in the transplanted testicles by AT-MSCs cultured in the growth factors-supplemented medium was greater than those in the control group (P<0.001 and P<0.05, respectively). The expression levels of the c-Kit and Scp3 genes did not significantly differ from the control group.
Our findings showed that the use of EGF, LIF and GDNF to culture AT-MSCs can be very helpful in terms of MSC survival and localization.