Characterization and In Silico Analysis of The Structural Features
of G-CSF Derived from Lysates of Escherichia coli
Granulocyte colony-stimulating factor (G-CSF) has a wide variety of functions including stimulation of hematopoiesis
and proliferation of granulocyte progenitor cells. Recombinant human G-CSF (rh-G-CSF) is used for treatment of neutropenia
in patients receiving chemotherapy. The mature bloodstream neutrophils express G-CSF receptor (G-CSFR), presenting
a significant and specific mechanism for circulating G-CSF clearance. Computational studies are essential bioinformatics
methods used for characterization of proteins with regard to their physicochemical properties and 3D configuration, as well
as protein–ligand interactions for recombinant drugs. We formerly produced rh-G-CSF in
Materials and Methods
In this experimental study, we analyzed the purified G-CSF using the analytical experiments. Then, the crystalline structure was extracted from Protein Data Bank (PDB) and molecular dynamics (MD) simulation was performed using Gromacs 5.1 package under an Amber force field. The importance of amino acid contents of G-CSF, to bind the respective receptor was also detected; moreover, the effect of dithiothreitol (DTT) used in G-CSF purification was studied.
The results revealed that characteristics of the produced recombinant G-CSF were comparable with those of the standard G-CSF and the recombinant G-CSF with the residual amino acid was stable. Also, purification conditions (DTT and existence of extra cysteine) had a significant effect on the stability and functionality of the produced G-CSF.
Experimental and in silico analyses provided good information regarding the function and characteristics of our recombinant G-CSF which could be useful for industrial researches.