Characterization and In Silico Analysis of The Structural Features of G-CSF Derived from Lysates of Escherichia coli


Sharareh Peymanfar, M.Sc, 1Rasoul Roghanian, Ph.D, 1,*Kamran Ghaedi, Ph.D, 1Sayed-Hamid Zarkesh-Esfahani, Ph.D., 1Reza Yari, Ph.D., 2
Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran
*Corresponding Address: P.O.Box: 81746 Department of Biology Faculty of Science University of Isfahan Isfahan Iran Email:r.roghanian@sci.ui.ac.ir
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Peymanfar Sharareh, Roghanian Rasoul, Ghaedi Kamran, Zarkesh-Esfahani Sayed-Hamid, Yari Reza. Characterization and In Silico Analysis of The Structural Features of G-CSF Derived from Lysates of Escherichia coli. Cell J. 2020; 21(4): 426-432.

Abstract

Objective

Granulocyte colony-stimulating factor (G-CSF) has a wide variety of functions including stimulation of hematopoiesis and proliferation of granulocyte progenitor cells. Recombinant human G-CSF (rh-G-CSF) is used for treatment of neutropenia in patients receiving chemotherapy. The mature bloodstream neutrophils express G-CSF receptor (G-CSFR), presenting a significant and specific mechanism for circulating G-CSF clearance. Computational studies are essential bioinformatics methods used for characterization of proteins with regard to their physicochemical properties and 3D configuration, as well as protein–ligand interactions for recombinant drugs. We formerly produced rh-G-CSF in E. coli and showed that the isolated protein had unacceptable biological activity in mice. In the present paper, we aimed to characterize the purified rh-G-CSF by analytical tests and developed an in vivo model by computational modelling of G-CSF.

Materials and Methods

In this experimental study, we analyzed the purified G-CSF using the analytical experiments. Then, the crystalline structure was extracted from Protein Data Bank (PDB) and molecular dynamics (MD) simulation was performed using Gromacs 5.1 package under an Amber force field. The importance of amino acid contents of G-CSF, to bind the respective receptor was also detected; moreover, the effect of dithiothreitol (DTT) used in G-CSF purification was studied.

Results

The results revealed that characteristics of the produced recombinant G-CSF were comparable with those of the standard G-CSF and the recombinant G-CSF with the residual amino acid was stable. Also, purification conditions (DTT and existence of extra cysteine) had a significant effect on the stability and functionality of the produced G-CSF.

Conclusion

Experimental and in silico analyses provided good information regarding the function and characteristics of our recombinant G-CSF which could be useful for industrial researches.