met1 DNA Methyltransferase Controls TERT Gene Expression: A New Insight to The Role of Telomerase in Development


Maryam Zangi, Ph.D., 1Mohammad Bagher Bagherieh Najjar, Ph.D., 1Masoud Golalipour, Ph.D., 2,*Mahnaz ghdasi, Ph.D., 1
Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran
Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran
Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran
* Corresponding Address: P.O.Box: 4934174515 Cellular and Molecular Research Center Golestan University of Medical Sciences GorganIran Email: gapmasood@goums.ac.ir
The Cell Journal (Yakhteh) is an open access journal which means the articles are freely available online for any individual author to download and use the providing address. The journal is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported License which allows the author(s) to hold the copyright without restrictions that is permitting unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective:

DNA methylation systems are essential for proper embryo development. Methylation defects lead to developmental abnormalities. Furthermore, changes in telomerase gene expression can affect stability of chromosomes and produces abnormal growth. Therefore, defects in both methylation and telomerase gene expression can lead to developmental abnormalities. We hypothesized that mutation in the methylation systems may induce developmental abnormalities through changing telomerase gene expression.

Materials and Methods:

In this experimental study, we used Arabidopsis thaliana (At) as a developmental model. DNA was extracted from seedlings leaves. The grown plants were screened using polymerase chain reaction (PCR) reactions. Total RNA was isolated from the mature leaves, stems and flowers of wild type and met1 mutants. For gene expression analysis, cDNA was synthesized and then quantitative reverse transcription PCR (qRT-PCR) was performed.

Results:

Telomerase gene expression level in homozygous met1 mutant plants showed ~14 fold increase compared to normal plants. Furthermore, TERT expression in met1 heterozygous was~ 2 fold higher than the wild type plants.

Conclusion:

Our results suggested that TERT is a methyltransferase-regulated gene which may be involved in developmental abnormities causing by mutation in met1 methyltransferase system.