met1 DNA Methyltransferase Controls TERT Gene Expression:
A New Insight to The Role of Telomerase in Development
Zangi M, Bagherieh Najjar MB, Golalipour M, Aghdasi M. met1 DNA methyltransferase controls TERT gene expression: a new insight to the role of telomerase in development. Cell J. 2020; 22(1): 71-74. doi: 10.22074/cellj.2020.6290.
DNA methylation systems are essential for proper embryo development. Methylation defects lead to developmental abnormalities. Furthermore, changes in telomerase gene expression can affect stability of chromosomes and produces abnormal growth. Therefore, defects in both methylation and telomerase gene expression can lead to developmental abnormalities. We hypothesized that mutation in the methylation systems may induce developmental abnormalities through changing telomerase gene expression.
Materials and Methods:
In this experimental study, we used Arabidopsis thaliana (At) as a developmental model. DNA was extracted from seedlings leaves. The grown plants were screened using polymerase chain reaction (PCR) reactions. Total RNA was isolated from the mature leaves, stems and flowers of wild type and met1 mutants. For gene expression analysis, cDNA was synthesized and then quantitative reverse transcription PCR (qRT-PCR) was performed.
Telomerase gene expression level in homozygous
Our results suggested that