The Effect of Melatonin on Mitochondrial Function and Autophagy
in In Vitro Matured Oocytes of Aged Mice
Nasheed Hamad Almohammed Z, Moghani-Ghoroghi F, Ragerdi-Kashani I, Fathi R, Tahaei LS, Naji M, Pasbakhsh P. The effect of melatonin on mitochondrial function and autophagy in in vitro matured oocytes of aged mice. Cell J. 2020; 22(1): 9-16. doi: 10.22074/cellj.2020.6302.
This study examined the
Materials and Methods
In this experimental study, germinal vesicles (GV) oocytes were collected from aged (with the
age of six-months-old) and young mice (with age range of 6-8 weeks old) and then cultured in the
The expression of SIRT-1 and LC3, as well as the measurement of the ATP content, was significantly increased in oocytes treated with melatonin compared with the oocytes receiving no treatment. Moreover, TAC was considerably higher in melatonin-treated oocytes than oocytes receiving no treatment. On the other hand, the level of ROS was significantly decreased in oocytes treated with melatonin in comparison with the untreated oocytes. The results indicated that melatonin considerably improved the development of oocytes as well.
According to the data, melatonin increased mitochondrial function and autophagy via an increase in the expression of SIRT1 and LC3, as well as the ATP contents while it decreased the levels of ROS and increased TAC in oocytes derived from aged mice.