Biosimilar Gene Therapy: Investigational Assessment of
Secukinumab Gene Therapy
Fallah A, Estiri H, Parrish E, Soleimani M, Zeinali S, Zadeh-Vakili A. Biosimilar gene therapy: investigational assessment of secukinumab gene therapy. Cell J. 2020; 21(4): 433-443. doi: 10.22074/cellj.2020.6309.
Tumor necrosis factor-alpha (TNF-α), checkpoint inhibitors, and interleukin-17 (IL-17) are critical targets in inflammation and autoimmune diseases. Monoclonal antibodies (mAbs) have a successful portfolio in the treatment of chronic diseases. With the current progress in stem cells and gene therapy technologies, there is the promise of replacing costly mAbs production in bioreactors with a more direct and cost-effective production method inside the patient’s cells. In this paper we examine the results of an investigational assessment of secukinumab gene therapy.
Materials and Methods
In this experimental study, the DNA sequence of the heavy and light chains of secukinumab
antibodies were cloned in a lentiviral vector. Human chorionic villous mesenchymal stem cells (CMSCs) were isolated and
characterized. After lentiviral packaging and titration, part of the recombinant viruses was used for transduction of the CMSCs
and the other part were applied for systemic gene therapy. The engineered stem cells and recombinant viruses were applied
Cell differentiation assays and flow cytometry of standard biomarkers confirmed the multipotency of the
CMSCs. Western blot and qRT-PCR confirmed
In this study, a lentiviral-mediated