Skin replacement has been a challenging task in wound healing resulted from burn. The application of laboratory based tissue expansion techniques is a potential solution to the problem of surface area cover. Fortunately, considerable progress has been made in approaches to allograft and autograft skin transplantation in order to replace skin temporarily or permanently. Despite of this progress, development of new treatments for burn victims are still a problem in cultured skin grafts. Hair follicles, sweat glands and other features of normal skin are absent in cultured skin. Scientists believe that Stem cells with unique characteristics including self renewal and differentiation potential offer a possible way for reconstruction of some structures within the wound. So, enhanced understanding of stem cell potentials may help develop novel therapies to overcome the problems in wound healing.