Electrospun Poly (ε-Caprolactone) Nanofiber Mat as Extracellular Matrix (Pages: )


Laleh Ghasemi-Mobarakeh , Mohammad Morshed *, Khadije Karbalaie , Mehrafarin Fesharaki , Mohammad Hossein Nasr-Esfahani *, Hossein Baharvand ,

Objective

Investigation of the suitability of electrospun Poly (ε-caprolactone) (PCL) nanofiber scaffold for the Vero cell culture.

MaterialsAndMethods

Electrospinning was used for production of PCL nanofibers scaffolds. Scanning electron microscopy (SEM), MTT assay, hematoxylin staining and histology analysis were used to investigate the cell morphology, viability, attachment and infilteration of the vero cells on the PCL nanofiber scaffolds.

Results

The results of the MTT assay, SEM images and hematoxylin staining showed that Vero cells attach and spread on PCL nanofiber scaffolds. The proliferation of Vero cells is as well as that of control group, but histological analysis showed the lack of cell infilteration into the scaffolds, which was found to be due to the small diameters of the pores of nanofibrous scaffold.

Conclusion

The result of this study show that PCL nanofiber scaffolds are suitable for cell culture, proliferation and attachment and Vero cells attach and proliferate on PCL nanofiber scaffolds.