The Synergistic Effect of Glucagon-Like Peptide-1 and Chamomile
Oil on Differentiation of Mesenchymal Stem Cells into
Saghahazrati S, Ayatollahi SAM, Kobarfard F, Minaii Zang D. The synergistic effect of glucagon-like peptide-1 and chamomile oil on differentiation of mesenchymal stem cells into insulin-producing cells. Cell J. 2020; 21(4): 371-378. doi: 10.22074/cellj.2020.6325.
Glucagon-like peptide-1 (GLP-1) has attracted tremendous attention for treatment of diabetes. Likewise, it seems that active ingredients of chamomile oil might have anti-diabetic effects. This work was conducted to investigate the effects of the combination of GLP-1 and chamomile oil on differentiation of mesenchymal stem cells (MSCs) into functional insulin-producing cells (IPCs).
Materials and Methods
In this experimental study, adipose MSCs derived from the adult male New Zealand white
rabbits were assigned into four groups: control (without any treatment); GLP-1 (in which cells were treated with 10 nM
GLP-1 every other day for 5 days); chamomile oil (in which cells were treated with 100 ug/ml
Our results demonstrated that isolated cells highly expressed MSC markers and were able to differentiate
into osteocytes and adipocytes. Additionally, using GLP-1 in combination with chamomile oil exhibited higher levels
of IPCs gene markers including NK homeobox gene 2.2 (
Collectively, these findings establish a substantial foundation for using peptides in combination with natural products to obtain higher efficiency in regenerative medicine and peptide therapy.