Incorporation of Silver Sulfadiazine into An Electrospun Composite
of Polycaprolactone as An Antibacterial Scaffold for
Wound Healing in Rats
Nejaddehbashi F, Hashemitabar M, Bayati V, Moghimipour E, Movaffagh J, Orazizadeh M, Abbaspour MR. Incorporation of silver sulfadiazine into an electrospun composite of polycaprolactone as an antibacterial scaffold for wound healing in rats. Cell J. 2020; 21(4): 379-390. doi: 10.22074/cellj.2020.6341.
Fabrication of an antibiotic-loaded scaffold with controlled release properties for wound dressing is one of tissue engineering challenges. The aim of this study was to evaluate the wound-healing effectiveness of 500-µm thick polycaprolactone (PCL) nanofibrous mat containing silver sulfadiazine (SSD) as an antibacterial agent.
Materials and Methods
In this experimental study, an electrospun membrane of PCL nanofibrous mat containing 0.3% weight SSD with 500 µm thickness, was prepared. Morphological and thermomechanical characteristics of nanofibers were evaluated. Drug content and drug release properties as well as the surface hydrophobicity of the nanofibrous membrane were determined. Antimicrobial properties and cellular viability of the scaffold were also examined. A full thickness wound of 400 mm2 was created in rats, to evaluate the wound-healing effects of PCL/SSD blend in comparison with PCL and vaseline gas used as the control group.
SSD at a concentration of 0.3% improved physicochemical properties of PCL. This concentration of SSD did
not inhibit the attachment of human dermal fibroblasts (HDFs) to nanofibers
Cytotoxicity of SSD in fabricated nanofibrous mat is a critical challenge in designing an effective wound
dressing that neutralizes cellular toxicity and improves antimicrobial activity. The PCL/SSD nanofibrous membrane with 500-
µm thickness and 0.3% (w/v) SSD showed applicable characteristics as a wound dressing and it accelerated wound healing