Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study

(Pages: 218-226)
Omid Zahedi Siani, M.Sc, 1Mahdi Sojoodi, Ph.D, 2,*Mohammad Zabetian Targhi, Ph.D, 1,*Mansoureh Movahedin, Ph.D., 3
Faculty of Mechanical Engineering, Tarbiat Modares University, Tehran, Iran
Faculty of Electrical and Computer Engineering, Tarbiat Modares University, Tehran, Iran
Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Faculty of Mechanical Engineering, Tarbiat Modares University, Tehran, Iran
Faculty of Electrical and Computer Engineering, Tarbiat Modares University, Tehran, Iran
Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
*Corresponding Addresses: P.O.Box: 14115-111 Faculty of Electrical and Computer Engineering Tarbiat Modares University Tehran Iran P.O.Box: 14115-111 Faculty of Mechanical Engineering Tarbiat Modares University Tehran Iran Emails:sojoodi@modares.ac.ir,zabetian@modares.ac.ir
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Zahedi Siani O, Sojoodi M, Zabetian Targhi M, Movahedin M. Blood particle separation using dielectrophoresis in a novel microchannel: a numerical Study. Cell J. 2020; 22(2): 218-226. doi: 10.22074/cellj.2020.6386.

Abstract

Objective

We present a four-branch model of the dielectrophoresis (DEP) method that takes into consideration the inherent properties of particles, including size, electrical conductivity, and permittivity coefficient. By using this model, bioparticles can be continuously separated by the application of only a one-stage separation process.

Materials and Methods

In this numerical study, we based the separation process on the differences in the particle sizes. We used the various negative DEP forces on the particles caused by the electrodes to separate them with a high efficiency. The particle separator could separate blood cells because of their different sizes.

Results

Blood cells greater than 12 μm were guided to a special branch, which improved separation efficiency because it prevented the deposition of particles in other branches. The designed device had the capability to separate blood cells with diameters of 2.0 μm, 6.2 μm, 10.0 μm, and greater than 12.0 μm. The applied voltage to the electrodes was 50 V with a frequency of 100 kHz.

Conclusion

The proposed device is a simple, efficient DEP-based continuous cell separator. This capability makes it ideal for use in various biomedical applications, including cell therapy and cell separation, and results in a throughput increment of microfluidics devices.