CD44 Gene rs8193 C Allele Is Significantly Enriched in Gastric Cancer Patients


Roya Mokhtarian, M.Sc, 1Hossein Tabatabaeian, Ph.D, 2,3Pardis Saadatmand, M.Sc, 4Mansoureh Azadeh, M.Sc, 4Negar Balmeh, M.Sc., 1Bagher Yakhchali, Ph.D., 5Kamran Ghaedi, Ph.D., 3,6,*
Division of Cellular and Molecular Biology, Department of Biology, NourDanesh Institute of Higher Education, Meymeh, Iran
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
ZistFanavari Novin Biotechnology Institute, Isfahan, Iran
Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology Institute, Tehran, Iran
Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Division of Cellular and Molecular Biology, Department of Biology, NourDanesh Institute of Higher Education, Meymeh, Iran
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
ZistFanavari Novin Biotechnology Institute, Isfahan, Iran
Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology Institute, Tehran, Iran
Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
*Corresponding Address: P.O.Box: 816513-1378 Department of Cellular Biotechnology Cell Science Research Center Royan Institute for Biotechnology ACECR Isfahan Iran Email:kamranghaedi@royaninstitute.org
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Mokhtarian Roya, Tabatabaeian Hossein, Saadatmand Pardis, Azadeh Mansoureh, Balmeh Negar, Yakhchali Bagher, Ghaedi Kamran. CD44 Gene rs8193 C Allele Is Significantly Enriched in Gastric Cancer Patients. Cell J. 2020; 21(4): 451-458.

Abstract

Objective

Gastric cancer is a multifactorial disease. In addition to environmental factors, many genes are involved in this malignancy. One of the genes associated with gastric cancer is CD44 gene and its polymorphisms. CD44 gene plays role in regulating cell survival, growth and mobility. The single nucleotide polymorphism (SNP) rs8193, located in the CD44 gene, has not been studied in gastric cancer patients of the Iranian population. The present study aims to study this polymorphism in 86 gastric cancer patients and 96 healthy individuals.

Materials and Methods

In this cross-sectional case-control study, rs8193 polymorphism was genotyped by allele specific primer polymerase chain reaction (ASP-PCR) technique. The obtained data were statistically analyzed. To find the potential mechanism of action, rs8193 was bioinformatically investigated.

Results

rs8193 C allele played a risk factor role for gastric cancer. Patients carrying this allele were more susceptible to have gastric cancer, with lymph node spread. On the other hand, rs8193 T allele, a protective factor, was associated with a higher chance of accumulation in the lower stages of cancer. C allele might impose its effect via destabilizing CD44 and miR-570 interaction.

Conclusion

rs8193 is statistically associated with the risk of malignancy, lymph node spread and stage of gastric cancer in Iranian population.