Chitosan Hydrogel Supports Integrity of Ovarian Follicles during
In Vitro Culture: A Preliminary of A Novel Biomaterial for
Three Dimensional Culture of Ovarian Follicles
Hassani F, Ebrahimi B, Moini A, Ghiaseddin A, Bazrafkan M, Hassanzadeh GH, Valojerdi MR. Chitosan hydrogel supports integrity of ovarian follicles during in vitro culture: a preliminary of a novel biomaterial for three dimensional culture of ovarian follicles. Cell J. 2020; 21(4): 479-493. doi: 10.22074/ cellj.2020.6393.
Testing novel biomaterials for the three dimensional (3D) culture of ovarian follicles may ultimately lead to a culture
model which can support the integrity of follicles during
Materials and Methods
In this interventional experiment study, CS hydrogels with the concentrations of 0.5, 1, and 1.5% were first tested for fourier transform infrared spectroscopy (FT-IR), Compressive Strength, viscosity, degradation, swelling ratio, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity and live/dead assay. Thereafter, mouse ovarian follicles were encapsulated in optimum concentration of CS (1%) and compared with those in alginate hydrogel. The follicular morphology, quality of matured oocyte and steroid secretion in both CS and alginate were assessed by enzyme-linked immunosorbent assay (ELISA). The expression of folliculogenesis, endocrine, and apoptotic related genes was also evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) and compared with day that in 0.
The rates of survival, and diameter of the follicles, secretion of estradiol, normal appearance of meiotic
spindle and chromosome alignment were all higher in CS group compared with those in alginate group (P≤0.05). The
The results showed that CS is a permissive hydrogel and has a beneficial effect on encapsulation of ovarian follicle and its further development during 3D culture.