Down-Regulation of miR-200c and Up-Regulation of miR-30c
Target both Stemness and Metastasis Genes in Breast Cancer
Rahimi M, Sharifi-Zarchi A, Zarghami N, Geranpayeh L, Ebrahimi M, Alizadeh E. Down-regulation of miR-200c and Up-regulation of miR-30c target both stemness and metastasis genes in breast cancer. Cell J. 2020; 21(4): 467-478. doi: 10.22074/cellj.2020.6406.
microRNAs (miRNAs) play important role in progression of tumorigenesis. They can target self-renewal and epithelial-mesenchymal transition (EMT) abilities in tumor cells, especially in cancer stem cells (CSCs). The objective of this study was to implement data mining to identify important miRNAs for targeting both self-renewal and EMT. We also aimed to evaluate these factors in mammospheres as model of breast cancer stem cells (BCSCs) and metastatic tumor tissues.
Materials and Methods
In this experimental study, mammospheres were derived from MCF-7 cells and characterized for the CSCs properties. Then expression pattern of the selected miRNAs in spheroids were evaluated, using the breast tumor cells obtained from seven patients. Correlation of miRNAs with self-renewal and EMT candidate genes were assessed in mammospheres and metastatic tumors.
The results showed that mammospheres represented more colonogenic and spheroid formation potential
than MCF-7 cells (P<0.05). Additionally, they had enhanced migration and invasive capabilities. Our computational
analyses determined that
Simultaneous down-regulation and up-regulation of respectively