Arsenic Trioxide and Thalidomide Combination Induces Autophagy
Along with Apoptosis in Acute Myeloid Cell Lines
Mohammadi Kian M, Haghi A, Salami M, Chahardouli B, Rostami Sh, Malekzadeh K, Kamranzadeh Foumani H, Mohammadi S, Nikbakht M. Arsenic trioxide and thalidomide combination induces autophagy along with apoptosis in acute myeloid cell lines. Cell J. 2020; 22(2): 193-202. doi: 10.22074/ cellj.2020.6469.
Autophagy and apoptosis play key roles in cancer survival and pathogenesis and are governed by specific genes which have a dual role in both cell death and survival. Arsenic trioxide (ATO) and thalidomide (THAL) are used for treatment of many types of hematologic malignancies. ATO prevents the proliferation of cells and induces apoptosis in some cancer cells. Moreover, THAL has immunomodulatory and antiangiogenic effects in malignant cells. The aim of present study was to examine the effects of ATO and THAL on U937 and KG-1 cells, and evaluation of mRNA expression level of VEGFs genes, PI3K genes and some of autophagy genes.
Materials and Methods
ATO/THAL combination enhanced cell apoptosis in a dose-dependent manner. Also, ATO/THAL induced SubG1/
G1 phase arrest. mRNA expression levels of
Combined treatment with ATO and THAL can inhibit proliferation and invasion of AML cells by down-regulating