Indirect Tumor Inhibitory Effects of MicroRNA-124 through
Targeting EZH2 in The Multiple Myeloma Cell Line
The first two authors equally contributed to this work.
Multiple myeloma (MM) is an incurable plasma cell malignancy. Several genetic and epigenetic changes
affect numerous critical genes expression status in this disorder.
Materials and Methods
In this experimental study, growth inhibitory effects of miR-124 were measured by MTT assay
in L-363 cell line. Likewise, cell cycle assay was measured by flowcytometery. The expression levels of
qRT-PCR results showed induction of
It seems that assembling of H3K27me3 mark mediated by EZH2 is one of the key mechanisms of suppressing CDKN2A gene expression in MM disease. However, this suppressive function is applied by a multi-factor mechanism. In other words, targeting EZH2, as the core functional subunit of PRC2 complex, can increase expression of the downstream suppressive genes. Consequently, by increasing expression of tumor suppressor genes, myeloma cells are stopped from aberrant expansions and they become susceptible to regulated cellular death.