Cytokine Gene Expression Alterations in Human Macrophages
Infected by Leishmania major
Leishmaniasis is caused by members of the
Materials and Methods
In this descriptive study, human monocytes were isolated by magnetic activated cell sorting
(MACS) and cultured in the presence of monocyte colony stimulating factor (M-CSF) to obtain the macrophages.
Monocyte-derived macrophages were then co-cultured with metacyclic promastigotes of
Our data revealed significant changes in immune response gene expressions in macrophages infected with L. major, with an up-regulation of cytokines and mostly down-regulation of their receptors.
The obtained data could shed more light on the biology of