Comparison of PLZF Gene Expression between Pluripotent
Stem Cells and Testicular Germ Cells
Azizi H, Koruji M, Skutella T. Comparison of PLZF gene expression between pluripotent stem cells and testicular germ cells. Cell J. 2020; 22(1): 60-65. doi: 10.22074/cellj.2020.6532.
Spermatogonial stem cells (SSCs), as unipotent stem cells, are responsible for the production of sperm
throughout the male’s life. Zinc finger and BTB domain containing 16 (
Materials and Methods
In this experimental study, we examined the expression of the PLZF germ cell marker in the testis section and testicular cell culture of neonate and adult mice by immunohistochemistry (IMH), immunocytochemistry (ICC) and Fluidigm Real-Time polymerase chain reaction (PCR).
IMH data indicated that the PLZF protein was localized in the neonate testis cells of the tubules center as
well as the basal compartment of adult testis seminiferous tubules. Counting PLZF IMH-positive cells in the sections
of seminiferous tubules of adult and neonate testis revealed significant expression of positive cells in adult testis
compared to the neonate (P<0.05). Under
These results indicate that PLZF is a specific transcription factor of testicular germ cell proliferation, but it is down-
regulated in pluripotent germ cells. This can be supportive for the analysis of germ cells development both