Young Researchers and Elite Club, Najafabad Branch, Islamic Azad University, Najafabad, Iran
Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Genetics, Faculty of Modern Medical Science, Islamic Azad University of Medical Sciences of Tehran, Tehran, Iran
Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran
Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
Department of Biology, Faculty of Natural Sciences and Mathematics, University of Tuzla, Tuzla, Bosnia and Herzegovina
Young Researchers and Elite Club, Najafabad Branch, Islamic Azad University, Najafabad, Iran
Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Genetics, Faculty of Modern Medical Science, Islamic Azad University of Medical Sciences of Tehran, Tehran, Iran
Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran
Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
Department of Biology, Faculty of Natural Sciences and Mathematics, University of Tuzla, Tuzla, Bosnia and Herzegovina
*Corresponding Address:
Department of Biology
Faculty of Natural Sciences and Mathematics
University of Tuzla
Tuzla
Bosnia and
Herzegovina
Email:amela.jusic@untz.ba
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Arshi A, Raeisi F, Mahmoudi E, Mohajerani F, Kabiri H, Fazel R, Zabihian-Langeroudi M, Jusic A. A comparative study of HOTAIR expression in breast cancer patient tissues and cell lines. Cell J. 2020; 22(2): 178-184. doi: 10.22074/cellj.2020.6543.
Recent data suggest that increased levels of the HOTAIR long non-coding RNA (lncRNA) are involved in
the development of various types of malignancy, including breast cancer. The aim of present study was to investigate
HOTAIR lncRNA expression profile in breast cancer (BC) patients and cell lines.
Materials and Methods
In this experimental study, expression level of HOTAIR lncRNA was evaluated in BC and
normal tissues of 15 patients as well as MDA-MB-231, MCF-7 and MCF-10A cell lines, using quantitative reverse-
transcription polymerase chain reaction (qRT-PCR). HOTAIR lncRNA expression levels were estimated using 2-ΔΔCt
method. Further, receiver operating characteristic (ROC) curve analysis was done to evaluate the selected lncRNA
diagnostic potential. The Cox’s proportional hazards regression model was performed to evaluate the predictive value
of this lncRNA level in BC patients.
Results
The results of present study demonstrated no significant difference in the expression of HOTAIR lncRNA in
MCF7 and MDA-MB-231 cancer cell lines compared to MCF-10A as normal cell line (P>0.05). However, we observed
a significantly increase in the expression of HOTAIR in BC patients compared to normal tissues (P<0.001). Significant
associations were found between gene expression and tumour size and margin. We found 91.1% sensitivity and 95.7%
specificity of circulating HOTAIR with an area under the ROC curve of 0.969. The Kaplan-Meier analysis indicated
significant correlation between HOTAIR expression and overall survival.
Conclusion
This study demonstrated that expression of HOTAIR is increased in BC and might be associated with its
progression. According to these findings, HOTAIR expression could be proposed as biomarkers for BC early diagnosis and
prognosis.
