The Effects of Embryonic Cerebrospinal Fluid on The Viability
and Neuronal Differentiation of Adipose Tissue-Derived
Stem Cells in Wistar Rats
Mohammadi-Mahdiabadi-Hasani MH, Nabiuni M, Parivar K, Yari S, Sahebi AR, Miyan J. The effects of embryonic cerebrospinal fluid on the viability and neuronal differentiation of adipose tissue-derived stem cells in wistar rats. Cell J. 2020; 22(2): 245-252. doi: 10.22074/cellj.2020.6560.
The embryonic cerebrospinal fluid (e-CSF) contains various growth factors and morphogens. Recent studies showed that e-CSF plays significant roles in embryonic brain development. Adipose tissue-derived stem cells (ADSCs) have a mesodermal origin that can be differentiated into mesodermal and ectodermal lineages. This study aimed to evaluate the effects of e-CSF on the proliferation, viability, and neural differentiation of ADSCs in rats.
Materials and Methods
In this experimental study, adipose tissue was dissected out from the inguinal region of adult
male rats. Then, ADSCs were isolated by enzymatic digestion from adipose tissues and mesenchymal cells were
confirmed using the flow cytometry analysis that measured the cell surface markers including CD90, CD44, CD73,
CD105, CD34, CD45, and CD11b. The multi-potential characteristics of ADSCs were assessed by osteogenic and
adipogenic potentials of these cells. Under suitable
The results of the present study demonstrated that the viability of ADSCs in cell culture conditioned with E17 and E18 e-CSF were significantly increased in comparison with controls. Cultured cells treated with e-CSF from E18 and E19 established neuronal-like cells bearing long process, whereas no process was observed in the control groups or cultured cells treated with E17 e-CSF.
This study showed that e-CSF has the ability to induce neuronal differentiation and viability in ADSCs. Our data support a significant role of e-CSF as a therapeutic strategy for the treatment of neurodegenerative diseases.