Investigation of VASA Gene and Protein Expression in Neonate and
Adult Testicular Germ Cells in Mice In Vivo and In Vitro
Azizi H, Ranjbar M, Rahaiee S, Govahi M, Skutella T. Investigation of VASA gene and protein expression in neonate and adult testicular germ cells in mice in vivo and in vitro. Cell J. 2020; 22(2): 171-177. doi: 10.22074/cellj.2020.6619.
We aimed to examine the expression levels of the
Materials and Methods
In this experimental study, in order to investigate the expression of this germ cell marker gene in more detail, we analyzed the expression of VASA by immunocytochemistry, immunohistochemistry and fluidigm reverse transcription-polymerase chain reaction (RT-PCR).
The immunohistochemical assays showed that the VASA protein was exclusively expressed in germ cells
in the seminiferous tubules of the neonate and adult testis and not in somatic cells. VASA was not detectable in
PLZF positive spermatogonial stem cells (SSCs), was weakly expressed in proliferating spermatogonia, and became
abundant in spermatocytes and round spermatozoa. Counting VASA-positive cells in the seminiferous tubules of the
neonate and adult testis depicted significant higher expression (P<0.05) of VASA in the adult testis in comparison
to its neonate counterpart. SSC colonies were established
The VASA protein is, therefore, an extremely specific marker of testicular germ cell differentiation