The Effect of Composol Medium on miR-16 Expression during Platelet Storage up to Day 7 at Room Temperature

(Pages: 542-548)
Ali Rajabi, Ph.D., Zohreh Sharifi, Ph.D., *Fatemeh Yari, Ph.D., Mohammadreza Deyhim, Ph.D., Mohammadali Jalili, Ph.D.,
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
*Corresponding Address: P.O.Box: 14665-1157 Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran Email:z.sharifi@ibto.ir
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Rajabi Ali, Sharifi Zohreh, Yari Fatemeh, Deyhim Mohammadreza, Jalili Mohammadali. The Effect of Composol Medium on miR-16 Expression during Platelet Storage up to Day 7 at Room Temperature. Cell J. 2021; 22(4): 542-547.

Abstract

Objective

MicroRNAs (miRNAs) are short, noncoding RNAs that play vital roles in gene regulation. It has been shown that storage has an effect on platelet miRNAs. MiR-16 is highly expressed in platelets and it appears to target the genes involved in cell death. It has been shown that platelets could be stored in Composol for a longer period of time. The aim of the present study was to assess and compare the expression pattern of miR-16 in platelet concentrates (PCs) in plasma and Composol media.

Materials and Methods

In an experimental study, ten PC bags were collected and each bag was divided into two separate bags, one with the 70% Composol and the other with only plasma. Both bags were stored for 7 days at 22˚C and tested on days 1, 3, 5, and 7 of storage. For each sample, we performed quantitative real-time polymerase chain reaction (qRT-PCR). The water-soluble tetrazolium salt-1 (WST-1) test was used to assess platelet viability in all of the samples. Statistical analysis was done by SPSS and REST software. A P<0.05 was considered statistically significant.

Results

miR-16 was significantly elevated during the storage days, with fold changes of 3.47 (plasma) and 2.77 (Composol). The Composol group had significantly decreased miR-16 expression compared with the plasma group. Results of the WST-1 test showed less decrease in optical density (OD) in the Composol group (0.93 ± 0.4) during the storage days compared with the plasma group (0.75 ± 0.3).

Conclusion

Our finding supported results from previous studies that reported an increase in miR-16 expression during platelet storage. In addition, miR-16 down-regulation in Composol medium implied that Composol might be a good solution for long-term platelet storage because it has the potential to elevate the shelf-life of platelets stored at 22˚C.