In this study, human primary fibroblast cells were exposed to static electromagnetic fields, and after transferring to a differentiated environment, the production of hematopoietic like stem cells in these cells was induced and tested.
After single layer cell formation, human primary fibroblast cells were treated with static electeromagnetic fields of 10 and 15 milli Tesla (mT) for 20 minutes each day for seven consecutive days. On the seventh day and immediately after the last radiation, the cells were added to the wells containing specific hematopoietic stem cell Expansion media. After 21 days and after colony formation, the cells of each group were evaluated for expressing CD34, CD38 and GATA-1 genes using Quantitative Real-Time PCR as well as surface marker expression of CD34 by flow cytometry.
Exposure to 10mT and 15mT magnetic fields increased the expression of CD34 and CD38 genes (P-value<0.05). This increase is 2.85 and 1.84 times, respectively in the 10mT group and 6.36 and 3.81 times, respectively in the 15MT group. The expression of GATA-1 gene in the 10mT and 15mT groups was not significantly different from that of the control group (P-value<0.05). Electromagnetic waves have caused the expression of CD34 marker at the surface of reprogrammed cells. This expression is about 42.3% in the 15mT group and 23.1% in the 10mT group.
The presence of human primary fibroblasts exposed to electromagnetic fields can increase the expression of specific hematopoietic genes. This method can be suitable for reprogramming cells differentiated into hematopoietic like stem cells and does not pose the risks of retroviral use.