Influence of Follicular Fluid and Seminal Plasma on The Expression
of Endometrial Receptivity Genes in Endometrial Cells
Endometrial receptivity plays a key role in pregnancy success in assisted reproduction cycles. Recent
evidence suggests that seminal plasma (SP) and follicular fluid (FF) influence the uterine endometrium to improve
implantation of the embryo and the establishment of pregnancy. In this study, we attempt to assess the influence of FF
and SP on the expression levels of main endometrial receptivity genes (
Materials and Methods
In this experimental study, SP and FF were collected from 15 healthy fertile men and 15 healthy fertile women, respectively. Tissue specimens of the endometrium were obtained from 12 women undergoing hysterectomy for benign conditions. After endometrial stromal cell isolation and culture, dose- and time-dependent cytotoxic effects of pooled FF and SP on 3D-cultured endometrial cells were evaluated. A second independent set of 12 endometrium samples was treated under determined optimum conditions and evaluated for gene expression analysis using quantitative real-time polymerase chain reaction (qRT–PCR).
The results of this study indicated that exposure of endometrial stromal cells to FF resulted in the elevated
Human SP and FF may modulate the endometrial receptivity and improve the implantation rate in assisted reproduction cycles through the up-regulation of endometrial receptivity genes.