Endoplasmic Reticulum Stress Induces miR-706, A Pro-Cell Death microRNA, in A Protein Kinase RNA-Like ER Kinase (PERK) and Activating Transcription Factor 4 (ATF4) Dependent Manner

(Pages: 394-400)
Xiu Wang, M.D., 1,#,*Yi Han, M.D. Ph.D, 2,#Guodong Hu, M.D, 2Jianbo Guo, M.D. Ph.D, 3Hongyu Chen, M.D, 2
Department of Anesthesiology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China
The Second Department of Urology, Shenyang Red Cross Hospital, Shenyang, People’s Republic of China (CHN)
The Third Department of General Surgery, The Fourth affiliated Hospital of China, Medical University, Shenyang, Liaoning, China
Department of Anesthesiology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China
The Second Department of Urology, Shenyang Red Cross Hospital, Shenyang, People’s Republic of China (CHN)
The Third Department of General Surgery, The Fourth affiliated Hospital of China, Medical University, Shenyang, Liaoning, China
*Corresponding Address: Department of Anesthesiology The Fourth Affiliated Hospital of China Medical University Shenyang Liaoning China Email:a6860330@yahoo.com

The first two authors equally contributed to this work.

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Wang X, Han Y, Hu G, Guo J, Chen H. Endoplasmic reticulum stress induces miR-706, a pro-cell death microRNA, in a protein kinase RNA-like ER kinase (PERK) and activating transcription factor 4 (ATF4) dependent manner. Cell J. 2020; 22(3): 394-400. doi: 10.22074/cellj.2020.6873.

Abstract

Objective

Endoplasmic reticulum (ER) stress causes an adaptive response initiated by protein kinase RNA-like ER kinase (PERK), Ire1 and ATF6. It has been reported that these upstream regulators induce microRNAs. The current study was designed to find a novel microRNA that mediates ER stress components and finally contributes to cell fate decision.

Materials and Methods

In this experimental study, miR-706 levels were checked under different conditions of ER stress induced by Thapsigargin, Tunicamycin or low glucose media. PERK and ATF4 were knocked-down by administration of lentivirus-mediated short hairpin RNA to explore the effect of ER stress related proteins on miR-706 expression. The effect of miR-706 on caspase activity and apoptosis inhibitor 1 (CAAP1) levels were examined by using mimic-miR-706. The role of CAAP1 in inhibiting cell death (measured by Annexin V staining) and contributing to patient overall survival (measured by Kaplan-Meier estimate) were further confirmed by anti- miR-706 and CAAP1 knock-down.

Results

We showed that Thapsigargin or Tunicamycin triggered ER stress leading to the induction of miR-706. miR-706 induction is dependent on PERK and its downstream regulator ATF4, as knocking-down of PERK and ATF4 suppressed miR-706 induction in response to ER stress. Knocking-down of miR-706 reduces cell death triggered by ER stress, indicating that miR-706 is pro-cell death microRNA. We further identified CAAP1 as a miR-706 target in regulating ER stress initiated cell death.

Conclusion

Collectively, our results pointed to an ER signaling network consisting of proteins, microRNA and novel target.