Enhancement of The Stability of Human Growth Hormone by Using Tris(hydroxymethyl)aminomethane: Molecular Docking and Experimental Analysis

(Pages: 406-414)
Siyavash Mirzaei, Ph.D, 1Hamid Mobedi, Ph.D, 2,*Hamid Gourabi, Ph.D, 1Mohammad Hosein Sanati, Ph.D, 3Sakine Khezli, M.Sc., 2Hamid Omidian, Ph.D., 4Masoume Ighaeie, Ph.D., 5
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Department of Novel Drug Delivery Systems, Iran Polymer and Petrochemical Institute, Tehran, Iran
Medical Genetics Department, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Department of Pharmaceutical Sciences, Nova Southeastern University, Pharmaceutical Sciences, Davie, Florida, USA
Computational Nano Physical Chemistry Laboratory, Department of Chemistry, Azerbaijan Shahid Madani University, Tabriz, Iran
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Department of Novel Drug Delivery Systems, Iran Polymer and Petrochemical Institute, Tehran, Iran
Medical Genetics Department, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Department of Pharmaceutical Sciences, Nova Southeastern University, Pharmaceutical Sciences, Davie, Florida, USA
Computational Nano Physical Chemistry Laboratory, Department of Chemistry, Azerbaijan Shahid Madani University, Tabriz, Iran
*Corresponding Address: P.O.Box: 14965-115 Department of Novel Drug Delivery Systems Iran Polymer and Petrochemical Institute Tehran Iran Email:h.mobedi@ippi.ac.ir
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Mirzaei Siyavash, Mobedi Hamid, Gourabi Hamid, Sanati Mohammad Hosein, Khezli Sakine, Omidian Hamid, Ighaeie Masoume. Enhancement of The Stability of Human Growth Hormone by Using Tris(hydroxymethyl)aminomethane: Molecular Docking and Experimental Analysis. Cell J. 2021; 22(4): 406-414.

Abstract

Objective

It is so difficult to formulate human growth hormone (hGH) in a solution with high stability and new drug delivery system (NDDs) due to physiochemical instability. The purpose of this study was to investigate the possibility of using Tris as a hGH stabilizer.

Materials and Methods

In this experimental study, the role of tris(hydroxymethyl)aminomethane (Tris) was evaluated as a hGH stabilizing agent in phosphate buffer, as a practical aqueous solution and a media to release NDDs. High- performance liquid chromatography (HPLC) and enzyme-linked immune sorbent assay (ELISA) were applied to investigate the stability of hGH in solutions and dynamic light scattering (DLS) was used to measure the effect of Tris on the hydrodynamic size of hGH in aqueous solutions. Ultra violet (UV) spectrophotometry was used to check the hGH spectrum. In computational study, formation of ligand-protein complex of the Tris-hGH, and the intermolecular interactions between Tris and hGH were studied by molecular docking modeling.

Results

The results demonstrated that Tris at the optimum concentration, increases hGH stability in aqueous solutions. Also, molecular docking modeling confirmed that amino acid residues such as tyrosine (Tyr), proline (Pro), glutamic acid (Glu), aspartic acid (Asp), leucine (Leu), and phenylalanine (Phe) in hGH structure, were linked with Tris as a ligand.

Conclusion

It seems that interactions between hGH and Tris are the most important reason for increment of the physicochemical stability of hGH in aqueous solutions containing Tris.