MYC Participates in Lipopolysaccharide-Induced Sepsis via Promoting Cell Proliferation and Inhibiting Apoptosis (Pages: 68-73)


Yin Li , Chengqi Kong , Lei Feng , Wenliang Tang , Mengwei Chen , Zhiyuan Zheng *,

Objective

This study aimed to explore the potential mechanism of MYC Proto-Oncogene, BHLH Transcription Factor (MYC) gene on sepsis.

MaterialsAndMethods

This is an experimental study. Rat-derived H9C2 cardiomyocyte cells cultured in vitro, followed by lipopolysaccharide (LPS) treatment with different concentration gradients. The cholecystokinin octapeptide (CCK-8) assay, enzyme-linked immunoassay (ELISA) assay, qRT-PCR, cell transfection, Western blot and flow cytometry were used to observe the cellular apoptosis and proliferation of cells in both LPS treatment groups and normal control group.

Results

The result of CCK-8 assay showed that silencing MYC inhibited cellular proliferation of sepsis with or without LPS treatment. ELISA assay showed that the expression of TNF-a and IL 6 were decreased in MYC silencing group, but increased after LPS treatment. Moreover, the Flow cytometry assay showed that MYC silencing contributed to the apoptosis of sepsis cells. Furthermore, the expression of inflammatory factors investigated showed that MYC silencing elevated the expression of inflammation factors.

Conclusion

MYC might take part in the process of LPS induced sepsis through suppressing apoptosis and inducing cell proliferation. Moreover, MYC might reduce inflammation during the progression of LPS induced sepsis.