Metastasis Inhibition by Cell Type Specific Expression of
BRMS1 Gene under The Regulation of miR200
Family Response Elements
Specific expression of therapeutic genes in cancer therapy has been per used for many years. One of the innovative strategies that have recently been introduced is employing miRNA response elements (MREs) of microRNAs (whose expression are reduced or inhibited in cancerous cells) into the 3´UTR of the therapeutic genes for their specific expression. Accordingly, MREs of anti-metastatic miRNA family have been used in 3´UTR of the metastasis suppressor gene in the corresponding cells to evaluate the level of metastatic behavior.
Material and Methods
In this experimental study, 3´UTR of the
It was shown that the employed MREs are able to up-regulate
It was demonstrated that a chimeric vector containing