Expression Change of miR-214 and miR-135 during Muscle Differentiation


Maryam Honardoost, M.Sc, 1,2,3Masoud Soleimani, Ph.D, 4Ehsan Arefian, Ph.D, 5Mohammad reza Sarookhani, Ph.D, 1,2,*
Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran
Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran
Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
*Corresponding Address: P.O.Box: 934197-59811 Department of Molecular Medicine School of Medicine Qazvin University of Medical Sciences Qazvin Iran Email:msarookhani@qums.ac.ir
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Abstract

Objective

MicroRNAs (miRNAs) are a class of small non-coding RNAs that play pivotal roles in many biological processes such as regulating skeletal muscle development where alterations in miRNA expression are reported during myogenesis. In this study, we aimed to investigate the impact of predicted miRNAs and their target genes on the myoblast to myocyte differentiation process.

Materials and Methods

This experimental study was conducted on the C2C12 cell line. Using a bioinformatics approach, miR-214 and miR-135 were selected according to their targets as potential factors in myoblast to myocyte differentiation induced by 3% horse serum. Immunocytochemistry (ICC) was undertaken to confirm the differentiation process and quantitative real-time polymerase chain reaction (PCR) to determine the expression level of miRNAs and their targets.

Results

During myoblast to myocyte differentiation, miR-214 was significantly down- regulated while miRNA-135, Irs2, Akt2 and Insr were overexpressed during the process.

Conclusion

miR-214 and miR-135 are potential regulators of myogenesis and are involved in skeletal muscle development through regulating the IRS/PI3K pathway.