Elevated Secretion of Aldosterone Increases TG/HDL-C Ratio and Potentiates The Ox-LDL-Induced Dysfunction of HUVEC

(Pages: 61-69)
Qian Zhang, Ph.D, 1Yiwen Pan, M.Sc, 2,*Xiaochun Ma, Ph.D, 1Hao Yang, B.Sc, 2Jun Chang, B.Sc, 2Ling Hong, M.Sc, 2Huiwen Yan, Ph.D., 2,*Shubing Zhang, Ph.D., 2,3,4,*
Department of Cardiovascular Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China
Department of Cell Biology, School of Life Sciences, Central South University, Changsha, Hunan, China
Hunan Key Laboratory of Animal models for Human Diseases, Central South University, Changsha, Hunan, China
Breast Cancer Research Center, School of Life Sciences, Central South University, Changsha, Hunan, China
Department of Cardiovascular Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China
Department of Cell Biology, School of Life Sciences, Central South University, Changsha, Hunan, China
Hunan Key Laboratory of Animal models for Human Diseases, Central South University, Changsha, Hunan, China
Breast Cancer Research Center, School of Life Sciences, Central South University, Changsha, Hunan, China
*Corresponding Address: Department of Cell Biology School of Life Sciences Central South University Changsha Hunan China Emails:yiwenpan@csu.edu.cn,huiwenyan1@hotmail.com,shubingzhang@csu.edu.cn
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Zhang Qian, Pan Yiwen, Ma Xiaochun, Yang Hao, Chang Jun, Hong Ling, Yan Huiwen, Zhang Shubing. Elevated Secretion of Aldosterone Increases TG/HDL-C Ratio and Potentiates The Ox-LDL-Induced Dysfunction of HUVEC . Cell J. 2021; 23(1): 61-69.

Abstract

Objective

Atherosclerosis (AS) is one of the most common causes of human death and disability. This study is designed to investigate the roles of aldosterone (Aldo) and oxidized low-density lipoprotein (Ox-LDL) in this disease by clinical data and cell model.

Materials and Methods

In this experimental study, clinical data were collected to investigate the Aldo role for the patients with primary aldosteronism or adrenal tumors. Cell viability assay, fluorescence-activated cell sorting (FACS) assay, apoptosis assay, cell aging analysis, and matrigel tube formation assay were performed to detect effects on human umbilical vein endothelial cells (HUVECs) treated with Aldo and/or Ox-LDL. Quantitative polymerase chain reaction (qPCR) and Western blot analysis were performed to figure out critical genes in the process of endothelial cells dysfunction induced by Aldo and/or Ox-LDL.

Results

We found that the Aldo level had a positive correlation with the TG/HDL-C ratio. Endothelial cell growth, angiogenesis, senescence, and apoptosis were significantly affected, and eNOS/Sirt1, the value of Bcl-2/Bax and Angiopoietin1/2 were significantly affected when cells were co-treated by Aldo and Ox-LDL.

Conclusion

Elevated Aldo with high Ox-LDL together may accelerate the dysfunction of HUVEC, and the Ox-LDL, especially for those patients with high Aldo should be well controlled. The assessment of the role of Aldo may provide a theoretical basis for the effective prevention and investigation of a new treatment of AS.