Identification of Circulating hsa-miR-324-3p and hsa-miR-331-3p
Exchanges in The Serum of Alzheimer’s Patients and Insights
into The Pathophysiological Pathways
Alzheimer’s disease (AD) is a type of dementia. Currently, there are not any existing and reliable methods
for the prognosis or diagnosis of AD. Hence, finding a diagnostic/prognostic biomarker for AD helps physicians to
prescribe the treatments and methods preventing disease progression. Circulating microRNAs (miRNAs) are the most
promising biomarkers due to their non-invasive and easily accessible for diagnosis and prognosis of AD. The aim of
current study is to evaluate expression levels of two unwell-known circulating miRNAs including
Materials and Methods
In this case and control study, to get the gene targets related to these two miRNAs, TargetScan, miRTargetLink Human and mirDIP web servers were applied. In addition, gene networks and gene ontology enrichment analysis were performed by STRING 10.5, KEGG and ShinyGO v0.41. Experimentally, expression levels of these two miRNAs in the serum of 21 patients with AD and 23 healthy individuals were compared using the quantitative reverse transcription polymerase chain reaction (qRT-PCR) method.
The pathophysiological pathways associated with these two miRNAs were nucleotide metabolism and cellular
response to stress pathway. Furthermore, the upregulated expression levels of
Non-significant results were obtained from the expression levels of AD patients and two significant pathways were obtained by networks and gene enrichment analysis.