Design and Microfabrication of An On-Chip Oocyte Maturation
System for Reduction of Apoptosis
(Pages: 32-39)
Abstract
Objective:
In customary assisted reproductive technology (ART), oocyte culture occurs in static micro drops of Petri
dishes with vast media volume; while, the
Materials and Methods:
In this experimental research, immature oocytes were collected from ovaries of the Naval
Medical Research Institute (NMRI) mice. Oocytes were randomly laid in static and dynamic (passive < active)
Results:
The MDA concentration in both dynamic oocyte maturation media were significantly lower than the static medium (0.003 and 0.002 vs. 0.13 µmol/L, P<0.01). Moreover, the rate of apoptosis in matured oocytes after a-24 hour maturation period was significantly lower in passive dynamic and active dynamic groups compared with the static group (16%, 15% vs. 35%, P<0.01).
Conclusion:
The dynamic culture for