Alpha-Lipoic Acid Can Overcome The Reduced Developmental
Competency Induced by Alcohol Toxicity during
Ovine Oocyte Maturation
Alpha-lipoic acid (ALA) as a strong antioxidant has a protective effect. This study was designed to assess
whether supplementation of maturation medium with ALA during
Materials and Methods
In this experimental study, to assess the antioxidant capacity of ALA challenged by 1% ethanol
The results revealed that alcohol significantly reduced cumulus cells (CCs) expansion index and blastocyst yield and rate of apoptosis in resultant embryos. Addition of 25 µM ALA to 1% ethanol during oocyte maturation decreased ROS level and elevated Thiolcontent. Furthermore, supplementation of maturation medium with ALA attenuated the effect of 1% ethanol and significantly increased the blastocyst formation and hatching rate as compared to control and ethanol groups. In addition, the quality of blastocysts produced in ALA+ethanol was improved based on the low number of TUNEL positive cells, the increased expression level of mRNA for pluripotency, and anti-oxidant markers, and decreased expression of apoptotic genes.
The current findings demonstrate that ALA can diminish the effect of ethanol, possibly by decreasing the ROS level and increasing Thiolcontent during oocyte maturation. Using the ALA supplement may have implications in protecting oocytes from alcohol toxicity in affected patients.