Alpha-Lipoic Acid Can Overcome The Reduced Developmental Competency Induced by Alcohol Toxicity during Ovine Oocyte Maturation

(Pages: 164-173)
Ali Moghimi Khorasgani, B.Sc, 1,2Reza Moradi, M.Sc, 1Farnoosh Jafarpour, D.V.M., Ph.D., 1,*Faezeh Ghazvinizadehgan, M.Sc., 1Somayyeh Ostadhosseini, D.V.M., 1Alireza Heydarnezhad, D.V.M., 1Ali Akbar Fouladi-Nashta, Ph.D., 3Mohammad Hossein Nasr-Esfahani, Ph.D., 1,*
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Department of Agricultural Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran
Reproduction Genes and Development Group, Department of Veterinary Basic Sciences, The Royal Veterinary College, HawksheadLane Hatfield, Herts AL97TA, UK
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Department of Agricultural Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran
Reproduction Genes and Development Group, Department of Veterinary Basic Sciences, The Royal Veterinary College, HawksheadLane Hatfield, Herts AL97TA, UK
*Corresponding Address: P.O.Box 8165131378 Department of Reproductive Biotechnology Reproductive Biomedicine Research Center Royan Institute for Biotechnology ACECR Isfahan Iran Emails:Jafarpour.farnoosh@gmail.com,mh.nasr-esfahani@royaninstitute.org
The Cell Journal (Yakhteh) is an open access journal which means the articles are freely available online for any individual author to download and use the providing address. The journal is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported License which allows the author(s) to hold the copyright without restrictions that is permitting unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Moghimi Khorasgani Ali, Moradi Reza, Jafarpour Farnoosh, Ghazvinizadehgan Faezeh, Ostadhosseini Somayyeh, Heydarnezhad Alireza, Fouladi-Nashta Ali Akbar, Nasr-Esfahani Mohammad Hossein. Alpha-Lipoic Acid Can Overcome The Reduced Developmental Competency Induced by Alcohol Toxicity during Ovine Oocyte Maturation. Cell J. 2021; 23(2): 164-173.

Abstract

Objective

Alpha-lipoic acid (ALA) as a strong antioxidant has a protective effect. This study was designed to assess whether supplementation of maturation medium with ALA during in vitro maturation (IVM) can attenuate the toxic effect of ethanol.

Materials and Methods

In this experimental study, to assess the antioxidant capacity of ALA challenged by 1% ethanol during in vitro maturation, immature ovine oocytes were exposed to 1% alcohol in the presence or absence of 25 µM ALA during oocyte maturation. The cumulus expansion index, intracellular reactive oxygen species (ROS), and thiol content levels were assessed in matured oocytes of various treatment groups. Consequently, the blastocyst formation rate of matured oocytes in various treatment groups were assessed. In addition, total cell number (TCN), cell allocation, DNA fragmentation, and relative gene expression of interested genes were assessed in resultant blastocysts.

Results

The results revealed that alcohol significantly reduced cumulus cells (CCs) expansion index and blastocyst yield and rate of apoptosis in resultant embryos. Addition of 25 µM ALA to 1% ethanol during oocyte maturation decreased ROS level and elevated Thiolcontent. Furthermore, supplementation of maturation medium with ALA attenuated the effect of 1% ethanol and significantly increased the blastocyst formation and hatching rate as compared to control and ethanol groups. In addition, the quality of blastocysts produced in ALA+ethanol was improved based on the low number of TUNEL positive cells, the increased expression level of mRNA for pluripotency, and anti-oxidant markers, and decreased expression of apoptotic genes.

Conclusion

The current findings demonstrate that ALA can diminish the effect of ethanol, possibly by decreasing the ROS level and increasing Thiolcontent during oocyte maturation. Using the ALA supplement may have implications in protecting oocytes from alcohol toxicity in affected patients.