MicroRNA and Hemophilia-A Disease: Bioinformatics Prediction and Experimental Analysis

(Pages: 341-348)
Halimeh Rezaei, Ph.D., 1Majid Motovali-Bashi, Ph.D., 1,*Seyed Javad Mowla, Ph.D., 2
Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, Iran
Molecular Genetics Department, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, Iran
Molecular Genetics Department, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
*Corresponding Address: P.O.Box: 8174673441 Department of Cell and Molecular Biology and Microbiology Faculty of Biological Sciences and Technology University of Isfahan Isfahan Iran Email:mbashi@sci.ui.ac.ir
The Cell Journal (Yakhteh) is an open access journal which means the articles are freely available online for any individual author to download and use the providing address. The journal is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported License which allows the author(s) to hold the copyright without restrictions that is permitting unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Rezaei Halimeh, Motovali-Bashi Majid, Mowla Seyed Javad. MicroRNA and Hemophilia-A Disease: Bioinformatics Prediction and Experimental Analysis. Cell J. 2021; 23(3): 341-348.

Abstract

Objective

Hemophilia-A is a common genetic abnormality resulted from decreased or lack of factor VIII (FVIII) pro-coagulant protein function caused by mutations in the F8 gene. Majority of molecular studies consider screening of mutations and their relevant impacts on the quality and expression levels of FVIII. Interestingly, some of the functions in FVIII suggest a probable involvement of small non-coding RNAs embedded within the sequence of F8 gene. Therefore, microRNAs which are encoded within the F8 gene might have a role in hemophilia development. In this study, miRNAs production in the F8 gene was investigated by bioinformatics prediction and experimental validation.

Materials and Methods

In this experimental study that approved by Research Assistant, University of Isfahan, bioinformatics tools have been utilized to seek the novel microRNAs inserted within human F8 gene. The ability to express new microRNAs in F8 locus was studied through reliable bioinformatics databases such as SSCProfiler, RNA fold, miREval, miR-FIND, UCSC genome browser and miRBase. Then, expression and processing of the predicted microRNAs were examined based on bioinformatics methods, in the HEK293 cell lines.

Results

We are unable to confirm existence of the considered mature microRNAs in the transfected cells.

Conclusion

We hope that through changing experimental conditions, designing new primers or altering cell lines as well as the expression of vectors, exogenous and endogenous expressions of the predicted miRNA will be confirmed.