Human Bone Mesenchymal Stem Cell-Derived Exosomes Inhibit
IL-1β-Induced Inflammation in Osteoarthritis Chondrocytes
Human bone marrow mesenchymal stem cell (hBMSC)-derived exosomes exhibit protective effects against
inflammatory diseases. This study aimed to explore the effects of hBMSC-derived exosomes on osteoarthritis (OA)
Materials and Methods
In this experimental study, we characterised exosomes derived from hBMSCs by transmission electron microscopy, nanoparticle tracking and Western blot analysis. Cellular uptake of exosomes was observed by fluorescent microscopy. Cell viability of chondrocytes exposed to interleukin-1 beta (IL-1β) was determined by the Cell Counting Kit-8 (CCK-8). Real-time quantitative polymerase chain reaction (RT-qPCR) was used to determine expression levels of genes related to apoptosis, inflammation, cartilage collagen metabolism and mitogen-activated protein kinases.
Fluorescence microscopy revealed that hBMSC-derived exosomes could be taken up by chondrocytes.
hBMSC-derived exosomes could significantly enhance cell viability of chondrocytes in response to IL-1β treatment.
RT-qPCR showed significant up-regulation of Survivin,
hBMSC-derived exosomes may play a protective role in chondrocytes through inhibiting cell apoptosis and the inflammatory response. These results will provide a novel therapeutic strategy for OA.